The relationship between biofilm formation and presence of fimH and mrkD genes among E. coli and K. pneumoniae isolated from patients in Mosul
Mosul Journal of Nursing,
2015, Volume 3, Issue 1, Pages 34-42
AbstractBackground and objectives: Biofilms have a major medical significance as they decrease susceptibility to antimicrobial agents. The decreased susceptibility to microbial agents within a biofilm arises from multiple factors, including physical impairment of diffusion of antimicrobial agents, reduced bacterial growth rates, and local alterations of the microor-ganisms (M.O) that may impair activity of the antimicrobial agent. The aim of the study was to investigate the biofilm formation among coliforms and study the relationship between biofilm formation and the presence of fimH and mrkD genes among E. coli and K. pneumonia isolates respectively .
Materials and method: One hundred and seventy three clinical samples were collected from both gender who visited or admitted to AL- Salam General Teaching Hospital and AL- Wafa’a center for diabetic patients in Mosul city from April 2013 to February 2014, (110) urine samples from patients suffering from urinary tract infections (UTIs) and (63 ) from patients with diabetic foot infections (DFIs). All samples were cultured on selective media as MacConkey and blood agar aerobically. Coliform isolates are identified to species level depending on biochemical and physiological tests and using Rapid ™ ONE panel kit to be conformed to the diagnosis. The ability of coliforms spp. for biofilm formation was assessed using qualitative and quantitative assay. The Congo red agar (CRA) method was used in the qualitative biofilm assay.
Results: Out of (85) bacterial spp. , 44(51.8%) species were biofilm producers. The tissue culture plate (TCP) method was used in the quantitative biofilm formation assay. The results showed that 46(54.1%) spp. produced biofilm strongly. All biofilm producing E. coli and K. pneumoniae spp. which gives strong biofilm formation by CRA method and non-biofilm producer spp. were subjected to PCR for determining the relation between biofilm formation and the presence of fimH gene in E. coli isolates and mrkD gene in K. pneumoniae isolates. The results revealed that all biofilm producing E. coli 15(100%) isolated from UTI samples were positive for fimH gene. While all biofilm producing K. pneumoniae 9(100%) and 5(100%) isolated from UTIs and DFIs respectively were positive for mrkD gene.
Agostinho, A.M.; Hartman, A.; Lipp, C.; Parker, A.E.; Stewart, P.S. and James, G.A. (2011). An in vitro model for the growth and analysis of chronic wound MRSA biofilms. J. Appli. Microbiol., 111: 1275-1282. Arabi, S.; Tohidi, F.; Naderi, S.; Nazemi, A.; Jafarpour, M. and Naghshbandi, R. (2012). The common fimbriae genotyping in uropathogenic Escherichia coli. Ann. Biolog. Res., 3(10): 4951-4954.
Banderia, M.; Carvalho, P.A.; D, A. and Jordao, L. (2014). Exploring dangerous connections between Klebsiella pneumoniae biofilms and healthcare-associated infections. Pathogens., 3: 720-731.
Bellifa, S.; Hassaine, H.; Balestrino, D.; Charbonnel, N.; Mhamedi, I.; Terki, I.K.; Lachachi, M.; Didi, W. and Forestier, C. (2013). Evaluation of biofilm formation of Klebsiella pneumoniae isolated from medical devices at the university hospital of Tlemcen, Algeria. Afr. J. Microbiol. Res., 7(49): 5558-5564.
Cergole-Novella, M.C.; Pignatari, A.C.C. and Guth, B.E.C. (2015). Adhesion, biofilm and genotypic characteristics of antimicrobial resistant Escherichia coli isolates. Braz. J. Microbiol., 46(1): 167-171.
Heydari, H.; Shokrollahi, M.R. and Movahedi, Z. (2013). Frequency of type 1 fimbriae among E. coli subtypes isolated from patients with urinary and gastrointestinal tract infection. Life Sci. J., 10(7): 578-582.
Hojati, Z.; Zamanzad, B.; Hashemzadeh, M.; Molaie, R. and Abolfazl, G. (2015). Detection of fimH gene in uropathogenic Escherichia coli strains isolated from patients with urinary tract infection. Jundishapur J. Microbiol., 8(2): 1-4.
Jagnow, J. and Clegg, S. (2003). Klebiella pneumoniae MrkD-mediated biofilm formation on extracellular matrix and collagen coated surfaces. Microbiology., 149: 2397-2405.
Jarjees, R.K. (2014). Detection of virulence factors and synergistic effect of some medical plants and antibiotics on uropathogenic Escherichia coli. Ph.D. Thesis. Salahaddin University . Erbil. Iraq.
Karimian, A.; Momtaz, H. and Madani, M. (2012). Detection of uropathogenic Escherichia coli virulence factors in patients with urinary tract infections in Iran. Afr. Microbiol. Res., 6(39): 6811-6816.
Lai, Y.M.; Norgainathai, R.; Zaw, M.T. and Lin, Z. (2015). A new primer set for detection of fimH gene in Escherichia coli isolates. Int. J. Collab. Res. Inter. Med. Pub. Heal., 7(4): 65-71.
Li, K.; Zhou, W.; Hong, Y.; Sacks, S.H. and Sheerin, N.S. (2009). Synergy between type 1 fimbriae expression and C3 opsonisation increases internalization of E. coli by human tubular epithelial cells.BMCMicrobiol.,9: 64
Madigan, M.T.; Martinko, J.M. and Parker, J. (2000). Brock biology of microorganisms. 9th ed. Prentice-Hall, Inc. Upper Saddle River, New Jersey. P. 785.
Melo, R.; Barros, E.M.; Loureiro, N.G.; Melo, H.R.; Maciel, M.A. and Lopes, A.C. (2014). Presence of fimH, mrkD, and irp2 virulence genes in KPC-2 producing Klebsiella pneumoniae isolates in Recife-PE, Brazil. Curr. Microbiol., DOI 10:1-8.
Moreira, C.G.; Palmer, K.; Whiteley, M.; Sircili, M.P.; Trabulsi, L.R.; Castro, A.F.P. and Sperandio, V. (2006). Bundle-forming pili and EspA are involved in biofilm formation by enteropathogenic Escherichia coli . J. Bacteriol., 188: 3952-3961.
Najar, G.A.; Nejad, M.M. and Mansouri, S. (2007). The comparison between virulence factors of Escherichia coli isolated from urinary tract infections and fecal flora. Res. Pharm. Sci., 2: 99-103.
Ong, C.-L.Y.; Beatson, S.A.; Totsika, M.; Forestier, C.; McEwan, A.G. and Schembri, M.A. (2010). Molecular analysis of type 3 fimbrial genes from Escherichia coli , Klebiella and Citrobacter species. BMC. Microbiol., 10(183): 1-12.
Poovendran, P. and Ramanathan, N. (2014). In vitro study on antibiotic susceptibility pattern of biofilm producing uropathogenic Escherichia coli isolates and their molecular characterization. Asian J. Pharm. Clin. Res., 7(3): 181-185.
Pramodhini, S.; Niveditha, S.; Umadevi, S.; Kumar, S. and Stephen, S. (2012). Antibiotic resistance pattern of biofilm-forming uropathogenes isolated from catheterized patients in Pondicherry, India. Austra. Med. J., 5(7): 344-348.
Stahlhut, S.G.; Struve, C. and Krogfelt, K.A. (2012). Klebsiella pneumoniae type 3 fimbriae agglutinate yeast in mannose-resistant manner. J. Med. Microbiol., 61: 317-322.
Swarna, S.R.; Madhavan, R.; Gomathi, S. and Thamaraiselvi, S. (2012). A study of biofilm on diabetic foot ulcer. Inter. J. Res. Pharma. Biomed. Sci., 3(4): 1809-1814.
Zubair, M.; Malik, A.; Ahmed, J.; Rizvi, M.; Farooqui, K.J. and Rizvi, M.W. (2011). A study of biofilm production by gram-negative organisms isolated from diabetic ulcer patients. Biolog. Med., 3(2):147-157.
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